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Coding

Part:BBa_K1333004:Design

Designed by: Huang Junxiang, Wang Xizi   Group: iGEM14_SYSU-China   (2014-09-24)


M13 bacteriophage g5p with a FLAG tag on C-terminal


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Between the g5p and FLAG coding sequencing, there are 9 nucleotides linker sequence coding a 3 amino acid peptide. It is not certain whether this peptide would affect the regular function of g5p protein.


Source

It is derived from the commercial M13KE vector(New England Biolabs) used in phage display system by PCR.

References